Background:

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Applications:

ELISA, WB

Name of antibody:

RIPK1

Immunogen:

Fusion protein of human RIPK1

Full name:

receptor interacting serine/threonine kinase 1

Synonyms:

RIP; RIP1; RIP-1

SwissProt:

Q13546

ELISA Recommended dilution:

5000-10000

WB Predicted band size:

76 kDa

WB Positive control:

HEPG2,Hela and K562 cell lysates

WB Recommended dilution:

500-2000