Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Applications:

WB, IHC, IF

Name of antibody:

NFKB2(Phospho-Ser866)

Immunogen:

Peptide sequence around phosphorylation site of serine 866(E-D-S(p)-A-Y) derived from Human NFκB-p100/p52.

Full name:

nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

Synonyms：

p52; p105; H2TF1; LYT10; CVID10; LYT-10; NF-kB2

SwissProt:

Q00653

IHC positive control:

Human breast carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

97 kDa

WB Positive control:

Human ovary cancer cells lysate

WB Recommended dilution:

500-1000

IF positive control:

Hela cells

IF Recommend dilution:

100-200