Background:

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Applications:

ELISA, WB

Name of antibody:

PFKFB3

Immunogen:

Synthetic peptide of human PFKFB3

Full name:

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Synonyms:

PFK2; IPFK2; iPFK-2

SwissProt:

Q16875

ELISA Recommended dilution:

5000-10000

WB Predicted band size:

60 kDa

WB Positive control:

293T,A549,A431,Hela and Jurkat cell lysates

WB Recommended dilution:

500-2000