Background:

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins. PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation.

Applications:

WB, IHC

Name of antibody:

PSME3

Immunogen:

Fusion protein of human PSME3

Full name:

proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)

Synonyms：

PSME3; Ki; PA28-gamma; PA28G; REG-GAMMA

SwissProt:

P61289

IHC positive control:

Human colon cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

30 kDa

WB Positive control:

MCF7 and PC12 cells, mouse spleen tissue

WB Recommended dilution:

500-2000