Background:

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Applications:

ELISA, WB, IHC

Name of antibody:

VRK1

Immunogen:

Fusion protein of human VRK1

Full name:

vaccinia related kinase 1

Synonyms:

PCH1; PCH1A

SwissProt:

Q99986

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human esophagus cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

45 kDa

WB Positive control:

Jurakt,Hela,293T and 231 cell lysates

WB Recommended dilution:

500-2000