Background:

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Applications:

WB, IHC

Name of antibody:

PRKAA1/PRKAA2(Phospho-Thr183/Thr172)

Immunogen:

Peptide sequence around phosphorylation site of threonine 183/threonine172(L-R-T(p)-S-C) derived from Human AMPKα1/AMPKα2.

Full name:

protein kinase AMP-activated catalytic subunit alpha 1/ protein kinase AMP-activated catalytic subunit alpha 2

Synonyms:

AMPK; AMPKa1; AMPK2; PRKAA; AMPKa2

SwissProt:

Q13131/P54646

IHC positive control:

Human lung carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

64 kDa

WB Positive control:

C2C12 cells lysates treated with serum or calf intestinal phosphatase(CIP)

WB Recommended dilution:

500-1000